TissueGen | ELUTE® Fiber – Biologics
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ELUTE® Fiber – Biologics

TissueGen®’s patented extrusion process occurs at room temperature enabling sensitive biologics to remain viable when loaded to fibers and delivered in controlled, sustained release profiles for the first time ever. With ELUTE fiber, TissueGen provides tailored release kinetics with retained biological activity.

TissueGen has extensive experience loading and delivering sensitive growth factors such as Nerve Growth Factor (NGF), Vascular Endothelial Growth Factor (VEGF), and other sensitive biological molecules including immune proteins and enzymes such as IgG and even live adenoviruses.

For example, ELUTE fibers delivering neurotrophic factors along a concentration gradient may selectively entice motor and sensory axons to cross a gap in a severed spinal cord by directing and upregulating axonal growth as depicted in vitro in the figure below.


When PC12 cells have been incubated with a physiologically relevant does of biologically active NGF, they sprout dendrites. As shown below, these cells exposed to ELUTE fiber loaded with NGF produce long, thin dendritic protrusions verifying the NGF’s retained bioactivity post loading. The photo below was taken 30 days after the start of the cell culture, thereby demonstrating the fiber’s ability to continuously elute NGF over time.

PC12 Figure 2 A

PC12 cells with no NGF present


B)PC12 cells in the presence of biologically active NGF released from a PDO fiber

PC12 cells in the presence of biologically active NGF released from a PDO fiber

TissueGen has successfully loaded the following classes of drugs:

  • Pharmaceuticals (antibiotics to antineoplastic)
  • Biologics:
    • Growth factors (BDNF, GDNF, NGF, VEGF)
    • Immune proteins and enzymes (Fab IgG, lysozyme)
    • Oligonucleotides to whole viruses

TissueGen extrudes and loads industry standard biodegradable polymers as well as proprietary polymers specified by customers.

Common polymers extruded include:

  • Poly(L-lactic acid) [PLLA]
  • Poly(p-dioxanone) [PDO]
  • Blends with poly(ε-caprolactone) [PCL]
  • Biopolymers (chitosan and silk)
  • Poly(L-lactic-co-glycolic acid) [PLGA]
  • Poly(D,L-lactic acid) [PDLLA]

ELUTE fiber loaded with biologics are ideal for a number of implantable
medical device applications, including:

  • Nerve regeneration
  • Regenerative medicine
  • Solid tumor remediation
  • Spinal cord repair
  • Dermal wound healing

Another example of retained biological activity with biologic-loaded ELUTE fiber was assayed with a human pancreatic tumor implanted in an immune compromised animal. After allowing the tumor to develop, an ELUTE fiber loaded with adenovirus for green fluorescent protein was implanted into the center of the tumor. As the figure to the right indicates, the explanted tumor contained a large region where nearly all the cells were green, thereby demonstrating that these cells were successfully transfected with the virus released from ELUTE fiber.


Learn more about the groundbreaking technology behind ELUTE fiber here.