ELUTE Fiber – Growth Factors
TissueGen’s ground breaking extrusion process occurs at room temperature, enabling many sensitive growth factors and biologically-based agents to remain viable when loaded to fibers for the first time ever. With its absorbable growth factor fibers, TissueGen provides tailored release kinetics with a high degree of retained biological activity.
TissueGen has extensive experience loading sensitive growth factors such as Nerve Growth Factor (NGF), Vascular Endothelial Growth Factor (VEGF), as well as other sensitive biological molecules including immune proteins and enzymes such as IgG and even live adenoviruses.
The following figure shows PC12 cells incubated with a strand of NGF-loaded fiber.
When PC12 cells have been incubated with biologically active NGF, they sprout dendrites. As shown above, the cells loaded with TissueGen’s fiber have long thin dendritic protrusions verifying the NGF’s retained bioactivity post loading. This photo was taken 30 days after the start of the cell culture experiment, thereby demonstrating the fiber’s ability to continuously elute NGF over time.
TissueGen’s growth factor fibers are ideal for a number of implantable medical device applications, including:
- Nerve regeneration
- Regenerative medicine
- Solid tumor remediation
- Spinal cord repair
- Dermal wound healing
In the following example of retained biological activity, a human pancreatic tumor was implanted in an immune compromised animal. After allowing the tumor to develop, a single TissueGen fiber loaded with adenovirus for green fluorescent protein was implanted into the center of the tumor. As the figure indicates, the explanted tumor contains a large region where nearly all the cells are green, thereby demonstrating that these cells were successfully transfected with the virus released from TissueGen’s fiber.